Insertion junction: CLIP2.058163_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.058163
Chromosome:chromosome 11
Location:353484
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467575 CLPB4 (1 of 1) PF07728//PF10431//PF13191 - AAA domain (dynein-related subfamily) (AAA_5) // C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small) // AAA ATPase domain (AAA_16); ClpB chaperone, Hsp100 family 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACTCCCCCAGCACCCATAACCGCGCCGCCACCCCCACACTCCACATCC
Internal bar code:CGTTGTATCTCATATCCTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1092
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGGAGGCGTCAGTTTCA
Suggested primer 2:CTCCACGGGCATGGTAGTTT