Insertion junction: CLIP2.058238_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.058238
Chromosome:chromosome 13
Location:4776426
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g604501 (1 of 1) IPR003613//IPR011050 - U box domain // Pectin lyase fold/virulence factor 3'UTR
Cre13.g604550 (1 of 8) PF14580 - Leucine-rich repeat (LRR_9) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACAACCGCCCGCGCCACAGCTACAACAGTCACCCCGCAATCGTGCTTC
Internal bar code:GTAGGTAAGGTCTTGTAGCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1039
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGATTGCTGCGTCGTTACAG
Suggested primer 2:ACAGTGCGAGTGACCAGATG