| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058375 |
| Chromosome: | chromosome 7 |
| Location: | 2476480 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329300 | MSC1 | Mechanosensitive ion channel; (1 of 1) PTHR30221 - SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL | 3'UTR |
| Cre07.g329350 | GT90F7,GT90-7 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 7 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGAGTTGCGCAAGGTCACACGATGTGAGCCCCCCCCCCCCGTCATGAA |
| Internal bar code: | GTTTACTCTTCGCACCAAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1521 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGCAGATGAAGGCGTTT |
| Suggested primer 2: | GACATCGAGTCGCTCATCGT |