Insertion junction: CLIP2.058401_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.058401
Chromosome:chromosome 11
Location:341077
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467575 CLPB4 (1 of 1) PF07728//PF10431//PF13191 - AAA domain (dynein-related subfamily) (AAA_5) // C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small) // AAA ATPase domain (AAA_16); ClpB chaperone, Hsp100 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTTTCCTCACCTGGGGTTCTCCGCCAGCGCCAGCACCATGTGCTCCA
Internal bar code:CGCCCCCAAAATGGAGATGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2170
LEAP-Seq percent confirming:98.2906
LEAP-Seq n confirming:115
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:117

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCCGATGCTGCACTTTCT
Suggested primer 2:TTGTACATGACGCGGCTCTT