Insertion junction: CLIP2.058760_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.058760
Chromosome:chromosome 3
Location:3527000
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g167550 POC3,CEP290 Proteome of centriole protein 3; (1 of 1) K16533 - centrosomal protein CEP290 (CEP290, NPHP6) 5'UTR
Cre03.g167600 CaM-IP3,FAP61 (1 of 1) PF16092 - Domain of unknown function (DUF4821) (DUF4821); Flagellar Associated Protein 61 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATGTGTTATGACGTAAATTGAAAATAAGATAACAGTCGTTAGTGACG
Internal bar code:TTAGTCTTGTGGTGATTTAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2782
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCGATATCTCACAGGCT
Suggested primer 2:CGGAACTCATAGTCGCCCTC