Insertion junction: CLIP2.058760_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.058760
Chromosome:chromosome 3
Location:3527003
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g167550 POC3,CEP290 Proteome of centriole protein 3; (1 of 1) K16533 - centrosomal protein CEP290 (CEP290, NPHP6) 5'UTR
Cre03.g167600 CaM-IP3,FAP61 (1 of 1) PF16092 - Domain of unknown function (DUF4821) (DUF4821); Flagellar Associated Protein 61 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAATCATTAAAGGGCTATAACAGCACAGTCGCACACTCGGTGCTCGTTC
Internal bar code:ATACCTCCGTGAATGATACGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3784
LEAP-Seq percent confirming:99.3151
LEAP-Seq n confirming:145
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:146

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAACTCATAGTCGCCCTC
Suggested primer 2:GCAGCGATATCTCACAGGCT