Insertion junction: CLIP2.058772_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.058772
Chromosome:chromosome 9
Location:2065320
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g396400 UBQ2 Bi-ubiquitin; (1 of 1) K12158 - ubiquitin-like protein Nedd8 (NEDD8) 5'UTR
Cre09.g396450 APT3,GOX15 Acid phosphatase; (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTCAACCCTTGTTGGACTTGGTGACTAGCAAAGATGCAGATTTTCGTC
Internal bar code:TGTATTTGACCCGCAGGGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:626
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCAATCCCCTTCCCCTC
Suggested primer 2:TGTGACTACGCCAGCAAGAG