| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058840 |
| Chromosome: | chromosome 10 |
| Location: | 1301956 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427150 | SPL16 | (1 of 1) K12870 - pre-mRNA-splicing factor ISY1 (ISY1); Pre-mRNA splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGCATGGGGTCTGGCACTTTGTTTCCTACCCCCCCCCTGGCAGATGG |
| Internal bar code: | ACGGGGAAGAACTGGATTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGAGTGGGTGTCGAATG |
| Suggested primer 2: | TGTGGTGCTTGCGTTTGATG |