| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.058856 |
| Chromosome: | chromosome 5 |
| Location: | 3095067 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755647 | (1 of 1) K14404 - cleavage and polyadenylation specificity factor subunit 4 (CPSF4, YTH1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCATGCACCCCCCCGGACAATTGTGCACATAACTTTTTGATGCCTA |
| Internal bar code: | AGTTAGGGTATCTGTGAGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1926 |
| LEAP-Seq percent confirming: | 78.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCGATAGTAGCGAGCAG |
| Suggested primer 2: | ATGCACCTGTCCCTCACATG |