Insertion junction: CLIP2.058878_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.058878
Chromosome:chromosome 11
Location:145569
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467539 GOX11,GOX6 (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase; Glyoxal oxidase 6 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCGTGACCTTGTGACCACTCATGCCAAGCCATGGAACCCTGGCATGG
Internal bar code:GGTGACGTGGCCAGCTCTTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2449
LEAP-Seq percent confirming:95.5556
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCCTTCATGCAGCAGTAC
Suggested primer 2:GTCTGCCAGTGAGTGTCGAA