| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.058997 |
| Chromosome: | chromosome 10 |
| Location: | 2743623 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438050 | KAS3,mtKAS | (1 of 1) PTHR11712//PTHR11712:SF273 - POLYKETIDE SYNTHASE-RELATED // 3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL; Putative mitochondrial beta-ketoacyl-ACP synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATAGCTGGTGCGCTGAGCGTGCATAGTGTAGCGATGATCCGCGCGGCC |
| Internal bar code: | AACTTCGTCGCTAACATGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3421 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTAGACGACCTAGCCTA |
| Suggested primer 2: | AGCAGCACATCTGAATGCCT |