| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.059479 |
| Chromosome: | chromosome 1 |
| Location: | 4018515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026400 | EXS1 | (1 of 1) PF03124 - EXS family (EXS); ERD1 homolog | 3'UTR |
| Cre01.g026450 | SRP35,SRS1 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 2) K12890 - splicing factor, arginine/serine-rich 1/9 (SFRS1_9) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATGTGCCTGTCTGCTACGGGTGAACCCCTTGGTCTCTGAATGAATTA |
| Internal bar code: | GTTTAAGTGGCTCAAGAGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3728 |
| LEAP-Seq percent confirming: | 95.1613 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTCAGTTGGCTTTGTGC |
| Suggested primer 2: | GGGAATCAGAATGGCCGACA |