| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060023 |
| Chromosome: | chromosome 17 |
| Location: | 957327 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702950 | GFY5 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTGCCCCTAGGAACAAAATAAAGAAACAAGCGAACCGTGAAGTGGTC |
| Internal bar code: | CGGGTTGTGGTCTCAGTTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1306 |
| LEAP-Seq percent confirming: | 46.4286 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGCTGCAGTAGGTGATA |
| Suggested primer 2: | GATGCACGATCTAGCGGACA |