| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.060023 |
| Chromosome: | chromosome 17 |
| Location: | 957391 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702950 | GFY5 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCAGAGTGTGTACGGTTTTATGACTGGGTGGGGGACACAGGACGGAT |
| Internal bar code: | TTAAAGTACATCGCTTTCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3924 |
| LEAP-Seq percent confirming: | 98.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCACGATCTAGCGGACA |
| Suggested primer 2: | AGGGGCTGCAGTAGGTGATA |