| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.060336 |
| Chromosome: | chromosome 12 |
| Location: | 7367367 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557650 | (1 of 100) IPR001810 - F-box domain | outside_mRNA | |
| Cre12.g557700 | (1 of 1) K01069 - hydroxyacylglutathione hydrolase (E3.1.2.6, gloB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAAGGATCCGAAGAATGATGTGTATGGCGGTGGTGCGTTCGAATGGG |
| Internal bar code: | AGTCGGGGACCGAAGGGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGATTGTCTGTGGAAGA |
| Suggested primer 2: | CAGAATGCAGAATGGAGCGC |