Insertion junction: CLIP2.060363_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.060363
Chromosome:chromosome 10
Location:2851011
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g439300 (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGCCTGCCGCCTACCTACGCGCCATCCGCCGCGCCGCAAGCAGCCCCT
Internal bar code:GGAAGGACGAGTTGGTTTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4848
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:TTCATCACGGTTCCTGCCTC
Suggested primer 2:AAAGGTAGAAAGAGCGGGGC