| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060435 |
| Chromosome: | chromosome 16 |
| Location: | 5392509 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683050 | RNJ1 | (1 of 1) 3.5.2.6 - Beta-lactamase / Penicillinase; chloroplast RNase J | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGATCCTGTGAGCCACAGCCGCGGATGCACGTCAACAAGGCAGCGGCG |
| Internal bar code: | GATGTCAGGACACAATGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCTACGCCTCGTCCTTC |
| Suggested primer 2: | ACGTTTGTGGAGTCGCTCAT |