Insertion junction: CLIP2.060612_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.060612
Chromosome:chromosome 6
Location:2718083
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g270850 SRH12 (1 of 1) K10841 - DNA excision repair protein ERCC-6 (ERCC6, CSB, RAD26); SNF2-related DNA/RNA helicase 5'UTR
Cre06.g270900 SUV3 Mitochondrial DEAD-box DNA/RNA helicase; (1 of 1) K17675 - ATP-dependent RNA helicase SUPV3L1/SUV3 [EC:3.6.4.13] (SUPV3L1, SUV3) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTAGAACAATACTGGGCAACTAGTACTTTGGAAGATTACTCGCAATGAT
Internal bar code:TCAACCAGTGTGGTGGTAAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1902
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCTACTGCTAATGCGTGTGC
Suggested primer 2:AACTTCCTTGCCGTAGCCTC