| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.060648 |
| Chromosome: | chromosome 3 |
| Location: | 4119477 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172600 | (1 of 1) IPR000104//IPR001841//IPR011990//IPR013083 - Antifreeze protein, type I // Zinc finger, RING-type // Tetratricopeptide-like helical domain // Zinc finger, RING/FYVE/PHD-type | 3'UTR | |
| Cre03.g172650 | BLD2,TUE1 | (1 of 1) K10391 - tubulin epsilon (TUBE); Epsilon tubulin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTATGGCTGTAGGTAGAAGTAGGACAGGGGCACTTAGATCATTGCCG |
| Internal bar code: | GAATGAGTATCCATCTGGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1709 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGAAACCTTGTTGCAGG |
| Suggested primer 2: | GATGAGGCGGACATACAGGG |