| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.060868 |
| Chromosome: | chromosome 3 |
| Location: | 4151643 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172950 | PUS1,PUS21,CBF5 | TruB family RNA pseudouridine synthase; (1 of 1) K11131 - H/ACA ribonucleoprotein complex subunit 4 (DKC1, NOLA4, CBF5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGCTCACGACGCGCTATGCGCACGCCGCAGCACCTCCTGCTCCCCGA |
| Internal bar code: | ACCAAGCCCAAGTTAAAACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1313 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGTCTTGCAGCGCGTTC |
| Suggested primer 2: | GAAGAGACCGTGTTTCCCGT |