| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.060948 |
| Chromosome: | chromosome 16 |
| Location: | 4049258 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690250 | PUS18 | TruB family RNA pseudouridine synthase; (1 of 1) K03177 - tRNA pseudouridine55 synthase [EC:5.4.99.25] (truB, PUS4, TRUB1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACTGTCACAGCAGTGAATGATGCTTACGAGAGCGTTGAACTAGAATC |
| Internal bar code: | AGGCGTCTGCGGGGCGTTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1546 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCAGCAGCTAAAGGAAT |
| Suggested primer 2: | CCCTCTGTTAGTGGCGAAGG |