Insertion junction: CLIP2.061179_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.061179
Chromosome:chromosome 1
Location:4095330
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g027150 CPLD46,HEL5 Conserved in the plant lineage and diatoms; (1 of 2) PTHR24031//PTHR24031:SF249 - RNA HELICASE // ATP-DEPENDENT DNA HELICASE RECG 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTCGCAGGGGCGAGGCTTCGGACGGCCGCAAGGGCGCGCTGCCGCAGC
Internal bar code:TTCACCGAGATGGCATCCACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:97
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGCGCGTGAAATAGTCG
Suggested primer 2:GTTGATGTGCTGCATAGGCG