Insertion junction: CLIP2.061267_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.061267
Chromosome:chromosome 9
Location:5924287
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g412000 (1 of 26) IPR013026//IPR019734 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGAATGAACACACCGTGCTCTTAACACGCGCTCGGCGAGGGAAACTAG
Internal bar code:AGCTATTATATTTCCGCTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2736
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTAGCCCGAATCCTAACG
Suggested primer 2:GAACAAAGCAGCTGGTGTGG