| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061523 |
| Chromosome: | chromosome 10 |
| Location: | 1446911 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428100 | TMG5 | (1 of 1) PTHR10631//PTHR10631:SF2 - N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE // N2, N2-DIMETHYLGUANOSINE TRNA METHYLTRANSFERASES-LIKE PROTEIN; possible N2,N2-dimethylguanosine tRNA methyltransferase | 3'UTR |
| Cre10.g428150 | (1 of 1) PTHR10566:SF58 - PROTEIN D2023.6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTATCCGCGTAAGAGTGGCGCCTGGGAACTCATCCAGGCGCTCGTGGC |
| Internal bar code: | GTGCTCCAGGGTCCAGAGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4181 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTGTGACAGGTTGCTA |
| Suggested primer 2: | AGTTTCCCCTGTGCTCGATG |