Insertion junction: CLIP2.061528_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.061528
Chromosome:chromosome 1
Location:821734
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g004550 FAP190 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase; Flagellar Associated Protein 190 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGAAATGCCACAATGACACCAGAAAACCGCGACACATCACCAGCACCG
Internal bar code:CGATTGTGCATTTACGACCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2570
LEAP-Seq percent confirming:85.2941
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:102

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGTGGAGGTGGTAATGG
Suggested primer 2:CATCCATATCCGGCGTGACA