| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061529 |
| Chromosome: | plastome |
| Location: | 65580 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802291 | ChreCp027,psbZ,2716976 | photosystem II reaction center Z protein; (1 of 1) K02724 - photosystem II PsbZ protein (psbZ) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACATCCTTTTGTAAATAGAACCTAAAAAGGAAAAAAATTGAATTTAA |
| Internal bar code: | TTTGGCTCTGATCGAGCTACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 31.25 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACTTTGGGGCTGCACAG |
| Suggested primer 2: | CCCTTCCGGGCAAGTAAACT |