| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.061810 |
| Chromosome: | chromosome 8 |
| Location: | 3198006 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376700 | MRCE1,MRC1 | (1 of 1) K13917 - mRNA-capping enzyme (RNGTT); mRNA capping enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTTTCCCGCTCCCCAGCTACGGGCTTACCCTGCCGTCCTTGGCTCT |
| Internal bar code: | CTGGGGTATACTAGTGTCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4577 |
| LEAP-Seq percent confirming: | 92.7273 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTGTAACGGGACTGTG |
| Suggested primer 2: | GAGGGTGTGATGGACAAGGG |