| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.061829 |
| Chromosome: | chromosome 16 |
| Location: | 3297049 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666334 | RFS1 | (1 of 1) 2.4.1.82 - Galactinol--sucrose galactosyltransferase / Raffinose synthase; putative raffinose synthase | intron |
| Cre16.g666400 | MAD1 | (1 of 1) K06638 - mitotic spindle assembly checkpoint protein MAD1 (MAD1L); putative mitotic spindle assembly checkpoint protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAGCCATGTATTTATTCAAAGGCCTTAGTTGTTCATTCCTTACTTGCA |
| Internal bar code: | TTGGTTCTGGCAAGAGCTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3612 |
| LEAP-Seq percent confirming: | 92.1569 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGTGCATTCAGCCTTC |
| Suggested primer 2: | GTGAGCTTGAGACCATGGCT |