Insertion junction: CLIP2.062090_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.062090
Chromosome:chromosome 3
Location:6009809
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g189650 (1 of 1) K04498 - E1A/CREB-binding protein (EP300, CREBBP, KAT3); Histone acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGTGGAGACTCGTGCAGCGCATAGACAGCTGCAGCATGAGCGAGTGA
Internal bar code:CATCCGGCGATACCGCACGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2816
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:AACTCTGCCGCATCATCCAA
Suggested primer 2:TGTAATGCGGAGATCGAGCC