| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.062144 |
| Chromosome: | chromosome 6 |
| Location: | 6184421 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291950 | MRS4,MRS3 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | intron |
| Cre06.g292000 | (1 of 1) PF00790//PF03127 - VHS domain (VHS) // GAT domain (GAT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAAGCTGCTGCGCACATCGTGGCCTCGCCTCCCCTTCTGCGGGTGCTG |
| Internal bar code: | TGACTAACAGGTGAACTTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGAGAGGGGAGCAAAAG |
| Suggested primer 2: | CAACTTTGACGACGAACCCG |