| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.062685 |
| Chromosome: | chromosome 1 |
| Location: | 5467670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038650 | SRS2 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 2) K12900 - FUS-interacting serine-arginine-rich protein 1 (FUSIP1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTATGCCAAGGCCCCTAAGTTACGTCAAGGCTGGGCTGGCCCGGCCCGA |
| Internal bar code: | TTGTACATGGTGTTGGGGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1703 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGCATGCGATTGCTTCAT |
| Suggested primer 2: | TGCCCGAGGAAGATCAGAGA |