| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.062786 |
| Chromosome: | chromosome 8 |
| Location: | 557215 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359650 | (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCTTAAACGCATGTAACGCTCCACCCCACACTACCCATGCGCACAA |
| Internal bar code: | TTTCGGCGCCTTTGTTCTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1110 |
| LEAP-Seq percent confirming: | 74.2424 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGCAACTCCATTACCCT |
| Suggested primer 2: | TTCACAGTAGCCCCGAACAC |