| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.063018 |
| Chromosome: | chromosome 3 |
| Location: | 3511550 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167500 | 3'UTR | ||
| Cre03.g167550 | POC3,CEP290 | Proteome of centriole protein 3; (1 of 1) K16533 - centrosomal protein CEP290 (CEP290, NPHP6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGACGATGCACCGCTACATGAAGCATTTGAGTGGCTTGGCCACTGT |
| Internal bar code: | TAGGCTGTGCCGGTGGGATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1838 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGTAAGGTTCCTGAGCA |
| Suggested primer 2: | TCGTGGGTTGAGGATTGGTG |