| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.063148 |
| Chromosome: | chromosome 3 |
| Location: | 4888640 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178600 | TOP1 | DNA topoisomerase I; (1 of 1) K03163 - DNA topoisomerase I [EC:5.99.1.2] (TOP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGAGGAAAGAGGGAGGTGCGGTGCAGGGGAGGGCGCATAACGGGAAAG |
| Internal bar code: | GCTTGAGATGCATTAACTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1874 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCTTGGTTCACTCGGCC |
| Suggested primer 2: | GAGCGGTTGTACGGATAGGG |