| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.063664 |
| Chromosome: | chromosome 3 |
| Location: | 128629 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143867 | 3'UTR | ||
| Cre03.g143887 | TSR1 | (1 of 1) 6.1.1.19 - Arginine--tRNA ligase / Arginyl-tRNA synthetase; Arginyl-tRNA Synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACAAGGGGTGGCCTGGCAGGAGTGATGAGGAAGAAAGTGGCTCTCC |
| Internal bar code: | GGCTACCTTTAGGCTGTGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 2.27273 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTCCATGGACAATTGAC |
| Suggested primer 2: | CATTGTACTGGACATGCGCG |