Insertion junction: CLIP2.063815_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.063815
Chromosome:chromosome 2
Location:2006528
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g088350 SNR3 (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc); SNARE associated Golgi protein 3'UTR
Cre02.g088400 DEG1,DEG1A (1 of 3) PTHR22939:SF81 - PROTEASE DO-LIKE 1, CHLOROPLASTIC; Deg protease 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTCTTACTTTCCCGCGTGTGCATTCCGCCAGCAGCGGTGACCTGCAAC
Internal bar code:ATCTGGACGGACCGAGGAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2494
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCATTCACTCCGCATTCG
Suggested primer 2:AAGGTCCGAGAGCAACCTTG