| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.064295 |
| Chromosome: | chromosome 6 |
| Location: | 3351381 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277801 | DNL4,DNL7 | DNA ligase; (1 of 2) K10777 - DNA ligase 4 (LIG4, DNL4) | 3'UTR |
| Cre06.g800655 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTTGATTCGCGCTCCAGGCTGCCCCGGGTTTTTTAGGGCTTCGCCAG |
| Internal bar code: | ATTAGGACGGAATTGTACAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3930 |
| LEAP-Seq percent confirming: | 98.4127 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAGAGCGGCCCTTAAGA |
| Suggested primer 2: | TGGGATGTTGTGTTAGCGCT |