| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064340 |
| Chromosome: | chromosome 1 |
| Location: | 4194302 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028200 | DBP2,HEL6 | (1 of 1) PTHR24031:SF219 - ATP-DEPENDENT RNA HELICASE DDX5-RELATED; DEAD-box RNA helicase | 5'UTR |
| Cre01.g028250 | GEA1 | Putative lysophospholipase, glycerol-ester acylhydrolase; (1 of 1) 3.1.1.23//3.1.1.3 - Acylglycerol lipase / Monoacylglycerol lipase // Triacylglycerol lipase / Triglyceride lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACTAAGCCAAGTCGCCGCACGTGCAGTGGTCAATCTTACCTCTCCAT |
| Internal bar code: | CCCCGAGCGATCAGATTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4918 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCACCACACACACACAC |
| Suggested primer 2: | TCCTCAAACGTCTTCACCGG |