| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064516 |
| Chromosome: | chromosome 2 |
| Location: | 7010870 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389652 | (1 of 1) PTHR31437//PTHR31437:SF1 - FAMILY NOT NAMED // PROTEIN SREK1IP1 | 5'UTR | |
| Cre09.g389689 | ASD1,ASSD1 | Aspartate semialdehyde dehydrogenase; (1 of 1) 1.2.1.11 - Aspartate-semialdehyde dehydrogenase / L-aspartate-beta-semialdehyde dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACGCCAAGCGGCGCTCCCCCAAGCGCCGGGTCCGGAATTATATTGGC |
| Internal bar code: | GCTTGTTAATGAGTGCCATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3045 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGGATTGCTGGTGATTG |
| Suggested primer 2: | CTGAGCTATGCCTCTGGTCG |