| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064661 |
| Chromosome: | chromosome 3 |
| Location: | 4889207 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178600 | TOP1 | DNA topoisomerase I; (1 of 1) K03163 - DNA topoisomerase I [EC:5.99.1.2] (TOP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGGGCAGCTAGCGTAGTTTAAGTGAATGCATGGCCCGGACAAGTTAC |
| Internal bar code: | CGACCCCTCGGTGTTCCTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3830 |
| LEAP-Seq percent confirming: | 34.7458 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 118 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACCAACCAGATGGAGA |
| Suggested primer 2: | CAAGTTCCCGGGTTGCTTTG |