Insertion junction: CLIP2.064917_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.064917
Chromosome:chromosome 2
Location:7014585
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389689 ASD1,ASSD1 Aspartate semialdehyde dehydrogenase; (1 of 1) 1.2.1.11 - Aspartate-semialdehyde dehydrogenase / L-aspartate-beta-semialdehyde dehydrogenase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTTGGTCAGCACAACTCCCGACTTGGAATAAACGGTTTACACGGAAGT
Internal bar code:CGAGGGAACGGATAATTCCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:3766
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACCACACACCACGTAAA
Suggested primer 2:GCTTCTTGCCAGCAGACCTA