| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.065239 |
| Chromosome: | chromosome 9 |
| Location: | 5015803 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405701 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGACTAGCAAGTGACGAAACGCGTGTTCTATGAATTAGCTCAGCTCA |
| Internal bar code: | GGTATGTCTAGCTTCCTCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCCAGTGCTGTACGAAG |
| Suggested primer 2: | ACGCATGCAAAACAGTGGTC |