| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065297 |
| Chromosome: | chromosome 12 |
| Location: | 1203739 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490250 | (1 of 1) PTHR12658//PTHR12658:SF0 - BETA-TUBULIN COFACTOR D // TUBULIN-SPECIFIC CHAPERONE D | 3'UTR | |
| Cre12.g490300 | MSD4 | (1 of 5) K04564 - superoxide dismutase, Fe-Mn family (SOD2); Mn superoxide dismutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGGAGCCATAGTTGCCATGATATTACACACCAGCCAAGCCAATTA |
| Internal bar code: | TCCCGAGCCGTGGCAGTGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 84.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCTCCTGGTCTCGGATT |
| Suggested primer 2: | TAAGGACAAGTGGGCTGCTG |