Insertion junction: CLIP2.065379_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.065379
Chromosome:chromosome 8
Location:1642769
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g365851 (1 of 2) 1.13.11.69//1.13.11.70 - Carlactone synthase // All-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase / Carotenoid cleavage dioxygenase 8 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAAGCAAACGTGCAATGCGAGTAGGGGGTGGGGGGGGAGTGTGGGGGC
Internal bar code:CTCGCGTCTAATTAATTTTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:349
LEAP-Seq percent confirming:3.125
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGTTGTGTGCTCAGCTG
Suggested primer 2:ATGAAGTGAAGTGCCGACGT