Insertion junction: CLIP2.065492_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.065492
Chromosome:chromosome 2
Location:3865527
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g101400 CHC1,NSG15 Clathrin Heavy Chain; (1 of 1) K04646 - clathrin heavy chain (CLTC) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGTGGAGCGTGAAGGAAGATTGCTGTAGACGCTAGAGCGGGCTCGCGG
Internal bar code:AGCGAAGTTGATTATGAAGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1412
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCACCAGGGCTGCAATAA
Suggested primer 2:CACGGCTCTGAGTCAAAGGT