| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065510 |
| Chromosome: | chromosome 16 |
| Location: | 7056039 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674515 | IDA7,DIC3,IA1-IC140,IC140 | (1 of 1) PTHR12442//PTHR12442:SF5 - DYNEIN INTERMEDIATE CHAIN // WD REPEAT-CONTAINING PROTEIN 63; Flagellar Inner Arm Dynein I1/f Intermediate Chain | 5'UTR |
| Cre16.g674627 | MKS1,B9D1 | (1 of 1) K16744 - B9 domain-containing protein 1 (B9D1); B9 Domain-Containing transition zone protein 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTTGAGCGCGAGACGAAGCTAGACGCACCTGGCTATCTTCTGCAATG |
| Internal bar code: | GTGGCCCACCTCATCGTGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2186 |
| LEAP-Seq percent confirming: | 94.6429 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGCGCCGTTTGTCCTG |
| Suggested primer 2: | AGTAGAAACGAGACGCGCAA |