Insertion junction: CLIP2.065513_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.065513
Chromosome:chromosome 17
Location:956942
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g702950 GFY5 (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTTGTCGGTATACCGCTGCACATCGCAATGGCCAAATGCCACTCACGCC
Internal bar code:CTTCTTCGTCCATGTGCAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1894
LEAP-Seq percent confirming:82.6087
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGTGTGCCACCTTCTGA
Suggested primer 2:GTCACCTTGCCCCTAGGAAC