| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066151 |
| Chromosome: | chromosome 16 |
| Location: | 1242401 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651050 | CYC6,PETJ | (1 of 2) K08906 - cytochrome c6 (petJ); Cytochrome c6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGTTGATGTTGTTATTTCAACTGGGTCACCGTAGCTAGCTCGTGCCC |
| Internal bar code: | TTGAAAGACATGATGTGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3926 |
| LEAP-Seq percent confirming: | 97.3333 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAATGGACTGCGATCGCC |
| Suggested primer 2: | CTTCAACGGCAACTGTGGTG |