| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066390 |
| Chromosome: | chromosome 1 |
| Location: | 792923 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004300 | ASNS,ASN1 | (1 of 1) K01953 - asparagine synthase (glutamine-hydrolysing) (asnB, ASNS); Asparagine synthase | 5'UTR |
| Cre01.g004350 | ROC105,ROC77,ALIX,NAT3,HLM1 | (1 of 1) K12200 - programmed cell death 6-interacting protein (PDCD6IP, ALIX, RIM20); Histone-lysine N-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTTATGTTAGCGATACATTGCAAGAAGACGGAGACTGTGGACATCAA |
| Internal bar code: | AGATTAAGGCGAGTGGTTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3106 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTCTCAATGGCGTTTAG |
| Suggested primer 2: | CGTCGAACAGGGCTAAACCT |