| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066532 |
| Chromosome: | chromosome 12 |
| Location: | 5176006 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526950 | (1 of 1) K03130 - transcription initiation factor TFIID subunit 5 (TAF5); Transcription initiation factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGCGGAGGGTGGAGGTCGGGCCCACCACTCTCCTGGACCTGACACGA |
| Internal bar code: | GCTAAGAAATTTGGTAAAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGGTGGCTTGTCTTGTA |
| Suggested primer 2: | CTGCGCTCTAGCTCCTACAC |